Course Syllabus
Organisation, Structure and Function of Genetic Material
1.1 Evidence of DNA as the genetic material
1.2 The composition and structure of DNA and RNA
1.3 The organization of DNA in chromosomes
1.4 Extrachromosomal genetic elements e.g. plasmid, transposon
DNA Replication
2.1 Principles of DNA replication (Models of DNA Replication)
2.2 Meselson & Stahl Experiment
2.3 Molecular model of DNA replication
2.4 DNA Polymerases: structure and function
Gene Expression: Transcription
3.1 Transfer of genetic information: the central dogma
3.2 Structure and function of mRNA
3.3 Transcription in prokaryotes
3.4 Transcription and RNA processing in eukaryotes
Gene Expression: Translation
4.1 Chemical and molecular structure of proteins
4.2 The nature of genetic codes
4.3 Ribosome structure and functions
4.4 Stages of translation
Recombination and Gene Transfer
5.1 Horizontal Gene Transfer (HGT)
5.2 Transformation
5.3 Generalised and specialised transduction
5.4 Conjugation
5.5 Significance of gene transfer
Regulation of Gene Expression
6.1 Overview of the regulation of gene expression in bacteria
6.2 Constitutive, inducible and repressible gene expression
6.3 Positive and negative control of gene expression
6.4 The Lactose operon in E. coli
6.5 The Tryptophan operon in E. coli
Special Topics in Microbial Genetics
7.1 Genome editing using CRISPR-Cas9 System
7.2 Antimicrobial Resistance
1.1 Evidence of DNA as the genetic material
1.2 The composition and structure of DNA and RNA
1.3 The organization of DNA in chromosomes
1.4 Extrachromosomal genetic elements e.g. plasmid, transposon
DNA Replication
2.1 Principles of DNA replication (Models of DNA Replication)
2.2 Meselson & Stahl Experiment
2.3 Molecular model of DNA replication
2.4 DNA Polymerases: structure and function
Gene Expression: Transcription
3.1 Transfer of genetic information: the central dogma
3.2 Structure and function of mRNA
3.3 Transcription in prokaryotes
3.4 Transcription and RNA processing in eukaryotes
Gene Expression: Translation
4.1 Chemical and molecular structure of proteins
4.2 The nature of genetic codes
4.3 Ribosome structure and functions
4.4 Stages of translation
Recombination and Gene Transfer
5.1 Horizontal Gene Transfer (HGT)
5.2 Transformation
5.3 Generalised and specialised transduction
5.4 Conjugation
5.5 Significance of gene transfer
Regulation of Gene Expression
6.1 Overview of the regulation of gene expression in bacteria
6.2 Constitutive, inducible and repressible gene expression
6.3 Positive and negative control of gene expression
6.4 The Lactose operon in E. coli
6.5 The Tryptophan operon in E. coli
Special Topics in Microbial Genetics
7.1 Genome editing using CRISPR-Cas9 System
7.2 Antimicrobial Resistance
Frequently Asked Questions
Q1 : Is there any laboratory session for this course?
A1 : Virtual lab for MOOC BMS424
Q2 : What will you learn in this course?
A2 : How to compare and contrast the mechanisms of DNA replication in prokaryotes and eukaryotes How to compare and contrast the mechanisms of gene expression in prokaryotes and eukaryotes How to describe gene expression and regulation in prokaryotes How to describe several mechanisms of Horizontal Gene Transfer (HGT) How to discuss the current trend and application of microbial molecular genetics
Q3 : Who is your instructor?
A3 : Dr Norashirene Mohamad Jamil (Ph.D) Senior Lecturer School of Biology, Faculty of Applied Sciences Universiti Teknologi MARA 40450, Shah Alam, Selangor. Alternative Email: [email protected] Mobile Contact: +6012-3043227 Expertise and interest - Molecular Biology, Microbiology, Microbial Genetics, Antibiotic Resistance, Transfer of Mobile Genetic Elements.
Q4 : Estimated duration of the course?
A4 : Estimated 8 weeks 3 hours per week
Q5 : Is there a pre-requisite for this course?
A5 : No, there is no requisite for this course
Q6 : What is the technology literacy needed for this course?
A6 : An individual's ability to assess, acquire and communicate information in a fully digital environment is expected. Digital literacy which can be defined as digital tool knowledge combined with critical thinking and social engagement skills are needed.
Q7 : What is expected of students?
A7 : Students are expected to be an independent active learner assisted with various study materials supported by the instructor. Students are encouraged to actively participate in group discussions and activities.
A1 : Virtual lab for MOOC BMS424
Q2 : What will you learn in this course?
A2 : How to compare and contrast the mechanisms of DNA replication in prokaryotes and eukaryotes How to compare and contrast the mechanisms of gene expression in prokaryotes and eukaryotes How to describe gene expression and regulation in prokaryotes How to describe several mechanisms of Horizontal Gene Transfer (HGT) How to discuss the current trend and application of microbial molecular genetics
Q3 : Who is your instructor?
A3 : Dr Norashirene Mohamad Jamil (Ph.D) Senior Lecturer School of Biology, Faculty of Applied Sciences Universiti Teknologi MARA 40450, Shah Alam, Selangor. Alternative Email: [email protected] Mobile Contact: +6012-3043227 Expertise and interest - Molecular Biology, Microbiology, Microbial Genetics, Antibiotic Resistance, Transfer of Mobile Genetic Elements.
Q4 : Estimated duration of the course?
A4 : Estimated 8 weeks 3 hours per week
Q5 : Is there a pre-requisite for this course?
A5 : No, there is no requisite for this course
Q6 : What is the technology literacy needed for this course?
A6 : An individual's ability to assess, acquire and communicate information in a fully digital environment is expected. Digital literacy which can be defined as digital tool knowledge combined with critical thinking and social engagement skills are needed.
Q7 : What is expected of students?
A7 : Students are expected to be an independent active learner assisted with various study materials supported by the instructor. Students are encouraged to actively participate in group discussions and activities.