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Course Info

About this Course

This course is designed to educate all students who are planning to start their journey in cell culture study. The content of the course will guide beginners on basic aseptic technique that is required in cell culture laboratory practice, the materials, consumables and instruments that are required for cell culture works and the basic steps in growing the cells for experimental use. The course will guide new student on how to start a cell culture experiment, media preparation, cell maintenance, cryopreservation, and common techniques that are being used in cell culture studies. In this course, common mistakes made by students and issues related to failure of experiments such as contamination will be highlighted and the steps to minimise the contamination will be addressed adequately. By joining the course, students will have a general overview on the correct steps in cell culture and should be confident in conducting their cell culture experiment independently.

Course Syllabus

History and Introduction of Cell Culture


Aseptic Technique


Equipments for Cell Culture


Chemical, Reagents and Media Used in Cell Culture


Preparation of Culture Media and Other Solutions


Culturing Cell Technique


Maintaining and Passaging the Cells


Cryopreservation of Cells


Cell Counting Method


Problems That May Arise and Troubleshooting


Frequently Asked Questions

Q1 : What is the knowledge that I need to have before joining MOOC Cell culture : From Theory to Practical?
A1 : There is no specific knowledge or requirement to join this MOOC. This MOOC is to educate all students who are planning to start their journey in cell culture study.