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Course Info

About this Course

This course is designed for preclinical medical students, to enhance the students' applied ability using virtual teaching tools and to improve their learning experience in the practical laboratory. It provides all materials for Biochemistry and Molecular Medicine practical under one official platform with self-assessment activities to assess the understanding of the online content. This is the first Biochemistry and Molecular Medicine MOOC practical that has been created under the Bachelor of Medicine-Bachelor of Surgery (MBBS) program. It is constructed according to MBBS current curriculum to provide effective learning experiences by including various activities such as quizzes, problem sets, and various engagements via peer and self-assessment, as well as online forums for discussion purposes.

Course Syllabus

Enzyme Activity
a) Determination of Alkaline Phosphatase (ALP) Activity
b) Determination of Km of the ALP
c) Determination of the effect of temperature on ALP activity
d) Determination of the effect of pH on ALP activity

Data Analysis in Enzymology
a) Introduction to data analysis in enzymology
b) Determination of Km and Vmax from Michaelis Menten graph and Lineweaver Burke graph
c) Differentiate the characteristics of Michaelis Menten enzyme and allosteric enzyme.
d) Factors affecting enzyme activity


DNA Extraction
a) Introduction to DNA extraction
b) DNA extraction at your own home!
c) Application of DNA extraction


PCR Applications
a) Part I: Polymerase Chain Reaction (PCR)
b) Part II: Gel electrophoresis and PCR product analysis
c) Applications of PCR

Data Analysis in Molecular Biology
a) Data analysis in molecular biology
b) DNA migration in gel electrophoresis
c) Restriction enzymes

Data Analysis on Lipoprotein
a) Introduction and lipoprotein electrophoresis principle
b) Interpretation of lipoprotein electrophoresis
c) Lipid profile

Urine Analysis
a) Introduction to urinalysis
b) Visual examination
c) Dipstick test
d) Microscopic examination

Glucose Tolerance Test and Anthropometry
a) Perform oral glucose tolerance test (OGTT)
b) Measure blood glucose level by using glucometer
c) Measure body mass index (BMI), waist-hip ratio and skinfold thickness

Frequently Asked Questions

Q1 : What is the knowledge that I need to have before joining the practical?
A1 : Students will be taught in a lecture for each topic depending on the module. The knowledge that students have learned during the lecture is adequate for a basic knowledge before joining the practical.