MOLECULAR DIAGNOSTIC AND CYTOGENETICS

About this Course

Course Description

This module aims to provide students with an understanding of the principles and applications of molecular diagnostics encompassing genomics and proteomics applicable in laboratory-based research projects and clinical diagnoses. It also covers the cytogenetics of humans by explaining the normal chromosomal structure and chromosome abnormalities. The methodology and techniques for the visualisation of chromosome aberrations used in clinical genetics and research are also taught.

Course Learning Outcomes

1 ) Describe the fundamentals and applications in genomics, proteomics and cytogenetics.
2 ) Demonstrate interactive conceptual frameworks in molecular diagnostics and cytogenetics as additional clinical diagnostic procedures.

Course Details

STATUS : Open
DURATION : FLEXIBLE
EFFORT : 2 hours per week
MODE : 100% Online
COURSE LEVEL : Intermediate
LANGUAGE : English
CLUSTER : Science & Technology ( ST )

 Syllabus

1. Nucleic acid extraction (genomic and plasmid DNA and RNA)
2. Quantification of nucleic acids: agarose gel electrophoresis, spectrophotometer, Lab-on-chip technology.
3. Gene amplification: PCR,
4. Gene expressions: Northern Blotting, Quantitative Real-time PCR, Microarray

1. Transformations.
2. Restriction Enzymes.
3. Gene Cloning.
4. Gene Sequencing.

1. Gel-based proteomics.
2. Antibody-based proteomics.
3. Mass-spectrometry proteomics.

1. Introduction to cytogenetics.
2. Structure of chromosomes.
3. Chromosome abnormalities.
4. Molecular cytogenetic techniques.

Our Instructor

DR. MAIMUNAH BT MUSTAKIM

Course Instructor
UiTM Kampus Puncak Alam

DR. NUR AYUNIE BINTI ZULKEPLI

Course Instructor
UiTM Kampus Puncak Alam

TS. DR. MOHD FADLY BIN MD AHID

Course Instructor
UiTM Kampus Puncak Alam

DR. EMIDA BT MOHAMED

Course Instructor
UiTM Kampus Puncak Alam

 Frequently Asked Questions

A1 : The practical session is only required for registered students of HS241 (Bachelor of Medical Laboratory Technology).